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> ニュース&トピックス 一覧 阪大院医 中田研講師らがAteloCell®マイティーを用いて、第9回 国際軟骨治療学会で「Certificate of Merit賞」を受賞!

ニュース&トピックス

阪大院医 中田研講師らがAteloCell®マイティーを用いて、
第9回 国際軟骨治療学会で「Certificate of Merit賞」を受賞!

2010年10月19日

この度、中田研講師(大阪大学大学院医学系研究科器官制御外科学)らの研究グループが、第9回 国際軟骨治療学会(the 9th World Congress of the International Cartilage Repair Society, ICRS)にて、AteloCell®コラーゲンスポンジマイティーを使用した研究のポスター発表で、「Certificate of Merit賞」を受賞されましたのでお知らせいたします。


学会名 the 9th World Congress of the International Cartilage Repair Society
会期 Sep.26-29, 2010
会場 Barcelona, Spain
受賞名 Certificate of Merit
演題 Prostaglandin E2 up-regulation by cyclic compressive loading on 3-D tissue of human synovium-derived stem cells and inhibitory effects of COX2 selective inhibitor or dexamethasone
発表者 K. Shimomura, T. Kanamoto, N. Nakamura, T. Mae, Y. Take, H. Kohda, S. Kuroda, Y. Akamine, K. Ota, H. Yoshikawa, K. Nakata
要旨 Purpose: Excessive mechanical stress on synovial joint causes osteoarthritis along with production of prostaglandin E2 (PGE2), a key molecule of arthritis, by synovial fibroblasts or chondrocytes. However, the molecular mechanism of PGE2 production by mechanical stress or the effects of NSAIDs or steroid is still unclear. The purpose of this study was to examine the expression of PGE2 by cyclic compressive loading on human synovium-derived mesenchymal stem cells (MSCs) and clarify the effects of NSAIDs or steroid.

Methods and Materials: Human synovium-derived MSCs were cultured onto collagen scaffolds to produce three-dimensional constructs. Cyclic compressive loading (40kPa, 0.5Hz, 1hr) was applied to the constructs with or without the administration of COX2 selective inhibitor or dexamethasone. After 6 hours incubation, the concentrations of PGE2, IL-1beta, TNF-alpha, IL-6, IL-8 in supernatant were measured by HTRF and the mRNA expressions for COX2 and mPGES-1 genes were examined by RT-PCR.

Results: The concentrations of PGE2, IL-6, and IL-8 in supernatant were significantly higher by mechanical stress, but those of IL-1beta and TNF-alpha unchanged. By administering COX2 selective inhibitor, the increased concentration of PGE2 by mechanical stress was impeded but those of IL-6 and IL-8 remained high. mRNA levels of COX2 and mPGES-1 genes were up-regulated by mechanical stress and this upregulation of COX2 was not suppressed by COX2 selective inhibitor, while that of mPGES-1 was suppressed. With dexamethasone, upregulation of PGE2, IL-6, and IL-8 was suppressed, and mRNA levels of COX2 and mPGES-1 were also suppressed.

Conclusions: Mechanical stress on the constructs up-regulated PGE2 production, while pro-inflammatory cytokines such as IL-1beta and TNF-alpha did not change. These results indicate that PGE2 up-regulation by mechanical stress is controlled not via these cytokines but via another signal pathway. COX2 selective inhibitor suppressed PGE2 production without changing COX2 mRNA level, while dexamethasone did by suppressing COX2 gene expression.



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