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ニュース&トピックス

第5回国際組織工学・再生医療学会世界会議2018にて学術発表

2018年8月10日

下記の要項にて学術発表を行います。


開催期間 2018年9月4日(火)〜9月7日(金)
9月5日(水)13:10〜14:10にポスター発表を行います。
会場 国立京都国際会館
演題 Micro-dimpled surface atelocollagen enhances the function of primary human hepatocytes
著者
(所属)
Tetsuro Sato, Kayoko Semura, Ichiro Fujimoto
(KOKEN Research Center, KOKEN Co. Ltd.)
要旨 Primary human hepatocytes (PHHs) are commonly used as the gold standard for drug development. However, maintaining functional PHHs in vitro has been difficult in conventional collagen coat culture. We developed a new scaffold using atelocollagen. The scaffold consists of higher amounts (mg/cm2 order) of atelocollagen, and it was exposed to ultraviolet radiation to induce cross-linking and improve stability. Observation using scanning and transmission electron microscopy revealed that the micro-dimpled surface (MDS) scaffold comprised randomly arranged atelocollagen fibrils. Thus, we named this collagen MDS atelocollagen. PHHs cultured on MDS atelocollagen were round with compact cytoplasm, and they exhibited enhanced albumin secretion levels and elevated cytochrome P450 (CYP)3A4 activity. The expression of hepatocyte-related genes such as serum proteins, drug metabolism-related CYPs and nuclear receptors was maintained in cells cultured on MDS atelocollagen but not in those cultured via conventional collagen culture. Therefore, our results suggest that MDS atelocollagen functionally enhances PHHs, conserving the usability of conventional PHHs collagen coat cultures.


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